Mitotimer ミトコンドリア

Author: rjni

August undefined, 2024

WebPlasmid pTRE-Tight-MitoTimer from Dr. Roberta Gottlieb's lab contains the insert MitoTimer and is published in Autophagy. 2013 Nov 1;9(11):1852-61. doi: 10.4161/auto.26501. Epub 2013 Oct 11. This plasmid is available through Addgene. WebmitoTimer体系：【将Timer基因与线粒体靶向序列结合，获得的MitoTimer蛋白能够在线粒体富集，实现对线粒体状态的检测。 Timer蛋白是红色荧光蛋白dsRed的突变体，其荧光 …

In vivo assay and modelling of protein and mitochondrial turnover ...

WebApr 25, 2014 · Mitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo. Here, we engineered a … WebOct 21, 2015 · Furthermore, individual mitochondria within cardiomyocytes display a higher red-to-green fluorescence, implying a block in import of newly synthesized MitoTimer … td bank republica dominicana

MitoTimer: a novel protein for monitoring mitochondrial

WebDec 14, 2024 · MitoTimer is a fluorescent protein targeted to the mitochondrial matrix. Upon translation, it emits green fluorescence, but after ∼16 h its emission shifts to red. Thus, the red: green ratio of cells expressing MitoTimer serves as an indicator for mitochondrial turnover, with an increase in the red: green ratio demonstrating a reduction in ... WebMitoTimer as a tool for monitoring mitochondrial turnover in vitro and in vivo. 3. Results and discussion 3.1. Constitutive expression of MitoTimer in vitro High membrane potential is required for mitochondrial protein import; thus hypo-polarized mitochondria cannot import newly syn-thesized (green) MitoTimer protein and consequently exhibit a high WebOct 21, 2015 · Furthermore, individual mitochondria within cardiomyocytes display a higher red-to-green fluorescence, implying a block in import of newly synthesized MitoTimer that would be caused by the lack of a high membrane potential, indicative of … bateria para x1 2019

MitoTimer: a novel tool for monitoring mitochondrial turnover

Conditional MitoTimer reporter mice for assessment of …

WebJan 31, 2024 · We demonstrate that the fluorescent protein, MitoTimer, is a reliable and robust probe to follow mitochondrial turnover. The screening of 15 000 small molecules led us to two chemically-related benzothiophenes that stimulate basal mitophagy in the beta-cell line, INS1. Enhancing basal mitophagy was associated with improved mitochondrial ... WebMay 18, 2024 · In transgenic Drosophila, MitoTimer has been used to visualize altered mitochondrial morphology and mitophagy in the heart and flight-muscle of flies with disrupted autophagy gene expression [Citation 100]. Finally, isotope labelling has been used to assess the rate of bulk mitochondrial protein synthesis. bateria para xbox oneWebMitotimer showed mitochondrial localization and changed in color from green to red over time. Scale bar: 20 µm. (B) Primary hippocampal neurons were transfected and imaged at the different time points after doxycycline induction. MitoTimer showed similar localization to mitochondria and kinetics of green to red transition as in MEF cells. bateria para xbox series

"WebOct 11, 2013 · Flow cytometry analysis of MitoTimer in cells. ( A) Transfected cells were exposed to Dox for 1 h, then cultured for the indicated times (4–144 h), harvested, and … " - Mitotimer ミトコンドリア

Mitotimer ミトコンドリア

Conditional MitoTimer reporter mice for assessment of …

WebJan 17, 2024 · A novel MitoTimer reporter gene for mitochondrial content, structure, stress, and damage in vivo. J Biol Chem 2014; 289: 12005–15. Crossref. PubMed. ISI. Google Scholar. 33. Stotland A, Gottlieb RA. alpha-MHC MitoTimer mouse: in vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. WebMitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo.Here, we engineered a MitoTimer reporter …

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WebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ... WebJan 1, 2024 · When CAG-CAT-MitoTimer mice were crossbred adiponectin-rtTA-TRE-Cre mice, the double transgenic offspring showed MitoTimer expression revealing mitochondrial network in white adipose tissue following 2 weeks of feeding with doxycline-chow (Fig. 2 e). Similarly, when crossbred with PepCK-Cre mice, the double transgenic offspring showed …

WebDec 6, 2014 · MitoTimer also provides information about the level of mitochondrial protein import across a population of cells and organelles. High membrane potential is required for mitochondrial protein import . Mitochondria which lose membrane potential will be unable to import new (green) MitoTimer protein. If biogenesis is impaired, one would expect a ... WebOct 11, 2013 · Flow cytometry analysis of MitoTimer in cells. ( A) Transfected cells were exposed to Dox for 1 h, then cultured for the indicated times (4–144 h), harvested, and …

WebConclusion: MitoTimer is useful for assessment of mitochondrial health in vivo. Significance: MitoTimer could advance mitochondrial research in multiple disciplines. Mitochondrial … WebPlasmid pTRE-Tight-MitoTimer from Dr. Roberta Gottlieb's lab contains the insert MitoTimer and is published in Autophagy. 2013 Nov 1;9(11):1852-61. doi: …

WebMay 1, 2016 · The MitoTimer transgene combines the red fluorescent protein, dsRed, and a human cytochrome c oxidase subunit VIII (Cox 8) mitochondrial localization sequence to traffic the protein to the mitochondria where the color shifts from green to red over time (approximately 24 hours). Our goal is to use this as a tool to assess the increase or ...

bateria para xbox series sWebMitoTimer expression was detected as early as 4 h and persisted (in the presence of continuous Dox) for at least 72 h. To overcome the asynchronous incorporation of … td bankruptcy\u0027sWebMitoTimer reporter gene. Mitochondria are the power plants in mammalian cells for ATP production essential for cellular processes. Mitochondrial dysfunction is associated with most of the prevalent health disorders, including cardiovascular disease, diabetes, cancer, and neurodegeneration. Impaired mitochondrial function often triggers a ... bateria para xiaomi mi 8 liteWebDec 1, 2015 · MitoTimer cDNA was introduced by blunt 5'end of the insert and the NheI-compatible site XbaI, ligated into a Mung Bean nuclease-blunted SalI site and the NheI site of the vector. MitoTimer transgenic mice were created in the C57/BL6 strain (Jackson Laboratories, Sacramento, CA) by pronuclear injection of murine alpha myosin heavy … td bank rio grande new jerseyWeb文献「MitoTimerは若いおよび老いたミトコンドリア蛋白質の細胞内分布および相対的ミトコンドリア蛋白質年齢に対するオートファジー,融合および運動能の効果を明らかにす … bateria para xiaomi g9WebMar 18, 2014 · Cox4 enrichment in the mitochondrial fraction and Gapdh in the cytosolic fraction indicate successful cell fractionation. d, expression of MitoTimer in a C. elegans touch neuron with mitochondria ... td bank st john\u0027s nlWeb(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria (lanes 1-3) and ... td bank saanich plaza